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The HPLC symposium series is a world-leading forum for the exchange of information among researchers involved in separations in liquid phases and supercritical fluids. The conference programme covers everything from fundamental and experimental approaches to column technologies and cutting‑edge applications and instrumentation. HPLC’s 2017 programme continues the trend of including separations coupled with mass spectrometry (MS), with one programme track now devoted solely to hyphenated techniques. Similarly, new trends, such as microfluidics and nanotechnologies, have been added. Vendors have always presented their latest technical innovations at the exhibition, which has always been an important part of the conference, and HPLC 2017 Prague will continue this tradition.