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The aim of this special issue is to keep track of new scientific accomplishments, to understand the availability of technologies, to appreciate the tremendous potential of AI in healthcare domain, and to provide researchers in related fields with inspiration. It can be asserted that, just like AI itself, the application of AI in biomedicine is still in its early stage. New progress and breakthroughs will continue to push the frontier and widen the scope of AI application, and fast developments are envisioned in the near future.