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After the great success of the first edition of this event, which took place in Porto in April 2022, we are delighted to welcome you to the 2nd Spring Biophotonics Conference. This year the location was changed to provide the participants with new experience in the northern coastal area of Portugal. The conference will be from 15 to 18 of June 2023 in the city of Espinho, which is located about 15 km south of Porto. The scope of this symposium ranges from fundamental research to translational research. It will cover all aspects from Bench to bedside to market. The goal of the OPORTO Conference is to bring together the key members of Biophotonics international community to present their latest research and to discuss future research plans, collaboration, exchange of ideas, plan visits to other labs and public engagement events. We hope that this event will bring such opportunities and that you will have an exciting and inspiring time. In this spirit of the scientific exchange, we would like to thank you in advance for your active contribution to making the OPORTO Conference a success. We hope that while you are experiencing the exciting atmosphere of this event, you will gain new insights about the fascinating new developments in the field. The meeting will cover specifically, but not limited to, light-tissue interaction, nano-photonics, neurophotonics, bioimaging, sensing and detection, multi-modal methods, theranostics and ethically aligned design of biophotonics instrumentation.