ИСТИНА

Hydrated Fullerene C60 is a 60 carbon atoms symmetrical molecule that can be found in nature. Being transferred into aqueous solution, a hydrophobic C60 establishes Hydrated Fullerene C60 (HyFnC60) water solutions that possess oxidation balancing properties in a wide range of concentrations. There is evidence of its ability to protect enzymes from oxidative inactivation and affect cell culture aging at low and ultra-low concentrations (2.5×10-6 – 2.5×10-19 М) [1, 2]. Nano-particles of silica (SiO2) can initiate reactive oxygen species (ROS) production in aqueous solutions. As silica is a common pollutant and a component of manufactured goods, one of the most probable ways of its penetration into human body is inhalation followed by an increased risk of chronic inflammation and cell degeneration [3-5] due to ROS production inside the cells and in the extracellular space [7-9]. In this study we focused on the possibility of DNA protection of human bronchial epithelial cells (HBEpCs) with HyFnC60 in a wide range of concentrations. HBECs in the medium were treated with 50 µg/ml silica particles. Then DNA strand breaks were accessed by comet assay. Intracellular ROS production was measured by DCFH–DA fluorescent spectrophotometry. HyFnC60 was added to the cell culture medium before treatment with silica. The calculated concentrations of HyFnC60 in the medium were 1×10-7 М 1×10-11 М, 1×10-14 М and 1×10-17 М. We have found that in case of pre-treatment of cells with 1×10-7 М and 1×10-17 М HyFnC60 prior addition of silica DNA damage was decreased. According to DCFH–DA fluorescent microscopy a significant decrease of ROS was observed in case of addition of HyFnC60 to the concentration of 1×10-7 М only. In case of simultaneous treatment of cells with silica and HyFnC60 cell damage was decreased at HyFnC60 concentration of 1×10-17 М.