ИСТИНА

Erythrocyte reversible aggregation and deformability are the major properties that affect blood microcirculation. Alterations in these properties lead to changing the blood viscosity and, as a consequence, to changes in blood flow through capillaries. This can lead to significant impairment of blood function, which leads to increasing a risk of occurrence of vascular concomitant diseases, and even the mortality especially in the case of cardiovascular pathologies. In this work, complex studies of the factors determining the capillary blood flow in patients suffering from such a socially significant disease as arterial hypertension were conducted by optical methods. Light scattering laser aggregometer and diffractometer Rheoscan (Rheomeditech, Korea) was used to conduct in vitro measurements of aggregation and deformability characteristics of the cells on ensembles of erythrocytes. Double-channeled optical tweezers were used for measuring the aggregation speed as well as interaction forces during erythrocyte doublet formation on cellular level. To quantitatively evaluate the capillary blood flow in vivo non-invasive capillaroscopy measurements in the nailfold vessels were conducted. In vitro measurements were performed with EDTA-stabilized human blood samples drawn from patients with arterial hypertension (AH) (70 people) and practically healthy volunteers – control (18 people). It was shown that in AH the ability of RBCs to deform is slightly reduced while the aggregation speed and forces of the cells interaction are significantly increased relative to the control group. In the case of AH, the blood microcirculation in nailfold capillaries is impaired as well. The work was supported by the grant of the Russian Science Foundation # 18-15-00422.