ИСТИНА

Mitochondrial uncoupling is known to decrease expression of some cell adhesion molecules (e.g. ICAM1, Intercellular Adhesion Molecule 1) in endothelial cells. We have tested the theory that this decrease is mediated via epigenetic changes in the promoter region of the ICAM1 gene. Genomic DNA was purified from endothelial cells incubated with various mitochondrial uncouplers of oxidative phosphorylation. The bisulfite-treated DNA was used for subsequent PCR, molecular cloning and sequencing. We have found a significant increase in CpG methylation level in the DNA of the cells treated with mitochondrial uncouplers. This work was supported by Russian Foundation for Basic Research, project №18-04-01110.