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Kara Sea copepods tend to accumulate trace elements. LIBS is well suited for their determination, but the lack of appropriate CRMs forces its application in a calibration-free mode. This study was focused on determination of ratios between Li, Na, K, Mg, Ca, Sr, and Al in zooplankton. This included plasma diagnostics and spectra modeling. The best results were obtained at early delays (0.5–0.75 μs). The reported study was funded by RFBR according to the research project № 18-33-01297.