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Protein engineering of fungal xylanases for bioprocessing of wood lignocellulosic feedstocksдоклад на конференции
- Авторы: Gusakov A.V., Rozhkova A.M., Denisenko Y.A.
- Международная Конференция : XVIII International Conference of Young Scientists “Forests of Eurasia – Serbian Forests”
- Даты проведения конференции: 24-28 сентября 2018
- Дата доклада: 24 сентября 2018
- Тип доклада: Пленарный
- Докладчик: Gusakov A.V.
- Место проведения: Белград, Serbia
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Аннотация доклада:
Site-directed mutagenesis of two fungal xylanases, PcXylA from Penicillium canescens and TrXyl3 from Trichoderma reesei, belonging to family 10 of glycoside hydrolases, was carried out in order to improve the enzyme thermostability. The most successful L18F mutant of PcXylA demonstrated an increase in the melting temperature by 4oC in comparison with the wild-type enzyme. As a result of insertion of five extra amino acid residues into the peptide loop forming the enzyme active site cleft, both xylanases acquired a resistance to XIP-type proteinaceous inhibitors, which reduce the enzyme efficiency in some biotechnological applications.
- Добавил в систему: Гусаков Александр Васильевич