ИСТИНА

Deformability is an essential intrinsic property of erythrocytes (red blood cells, RBC) which allows them to reversibly change shape and linear sizes and realize the gas transport function. RBC deformability is determined by cells internal viscosity and cytoskeleton structure. Understanding molecular mechanisms of the RBC deformability regulation according to the metabolic demands of the organism is an important task required for an effective control of the blood microrheology. We studied the effect of activation of RBC membrane protein adenylyl cyclase on RBC deformability. This process may play a key role in the receptor-mediated stimulation of signaling pathways leading to the conformational changes of RBC cytoskeleton proteins. We implemented laser ektacytometer RheoScan AnD-300 to assess changes of deformability of RBC extracted from heparinized blood at presence of forskolin – the direct stimulator of adenylyl cyclase.