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Magnetotactic spirillum was isolated from freshwater sediments of Lake Beloe Bordukovskoe (Moscow region). Strain LBB-42 biomineralizes the cuboctahedralmagnetite magnetosomes 38 nm in size. Phylogenetic analysis of 16S rRNA gene revealed that new strain has a maximum identity with cultivated spirillum Magnetospirillum marisnigri SP-1 T(97.1%). The genome of newly isolated bacteria was sequenced, it consists of4411256 bp in 69 contigs with average G+C content of 63.4%. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) with closely related genomes of Magnetospirillum strain SP-1 were computed and identity appeared to be 83.12% and 25.2% respectively. On the basis of this evidences the newly isolated strain was designated as novel species of Magnetospirillum genus for which was proposed the name Magnetospirillum kuznetsovii LBB-42.