ИСТИНА

We demonstrate multimodal optical imaging framework that combines single-neuron fluorescence microscopy with a variety of nonlinear-optical imaging techniques, including two-photon fluorescence, second- and third-harmonic generation, as well as coherent and stimulated Raman scattering. This provides a unique arsenal of tools for single-cell imaging of neurons expressing opto- and thermogenetic channels and/or fluorescent reporters in neuronal cultures, ex vivo brain slices, and precisely targeted regions inside the brain of awake transgenic mice and within the nervous system of zebrafish models.