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Microbial fuel cell (MFC) is a new technology that uses microorganisms to extract energy from complex organic mixtures. On the basis of aerobic granular sludge we have selected a nitrogen-fixing community of microorganisms that was immobilized on a carbon material (graphite foam, carbon nanotubes). The MFC anode has been developed on the basis of selected biological material. A membraneless glucose / oxygen MFC with bioanode and cathode based on non-platinum group metals or laccase enzyme has been developed. A mathematical model describing the processes in the MFC has been developed, on its base the calculations have been carried out.