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In the present work the constructions of solid-phase optical biosensors for the determination of different biologically active compounds (such us phenolic compounds, catecholamines, dibenzothiophenes and products of their oxidation, organic peroxides) with photometric, fluorescent and Surface-Enhanced Raman Spectroscopy (SERS) detection of analytical signal have been developed. The action of the proposed biosensors is based on the molecular recognition of the above mentioned analytes by the enzyme - horseradish peroxidase, HRP (or hemoglobin from bovine blood, Hb), which are included into a self-assembled universal optical transparent film of a biopolymer - chitosan.