ИСТИНА

Spherical lipid bilayer vesicles (liposomes) are widely used for delivery of biological active substances. The main advantage of such vesicles is the possibility to encapsulate either hydrophilic compounds into the inner water cavity, or the hydrophobic into the liposomal membrane. The biomedical application is maintained by the fact that the liposomes are not toxic because the lipids are the components of cell membrane. We demonstrate that electrostatical adsorption of anionic small unilamelar liposomes composed of mixtures of synthetic lipids (anionic and zwitterionic) on the surface of polycationic star-shaped polymer results in formation of nanocarrier barring tens of liposomes in small volume. This allows one not only increase the effective volume of liposomal nanocontainer but opens the new way for formation of multi-drug systems with desired composition. Traditional targets for liposomal nanocontainers are surrounded by media with locally changed parameters such as pH (tumors), temperature (inflammations), concentration of multivalent ions (Wilson`s disease) etc. Incorporation of stimuli-responsive compounds in lipid membrane such as pH-sensitive or Cu2+-sensitive lipid results in formation of liposomes with controlled release properties. Adjusting of membrane composition allows one to control the rate and efficiency of release of encapsulated substances. For the first time we demonstrate application of liposomes with membrane in gel state as perspective nanocontainers. Modification of polycationic stars with liposomes results in lowering of their cytotoxicity. Thus such complexes are promising for creation of biocompatible multi-drug nanocarrier with controlled release properties. Acknowledgement This work was supported by Russian Foundation for Basic Research projects 15-33-20880 and 16-33-01083)