ИСТИНА

Beverage quality control for contaminants such as dyes, additives, pesticides, mycotoxins and many others compounds are a serious and growing problem. The method for detection of contaminants must be simple, quick and cheap. Moreover, the analysis must be with high-throughput screening (HTS). The current methods of assays like chromatography techniques and ELISA have several limitations for HTS. Fluorescence Polarization Immunoassay (FPIA) could be the more optimal method for screening of food samples for contaminants. The FPIA is homogeneus method based on the competitive binding and the measurement of fluorescence polarization value for reaction mixture of sample (typically 10-50 µl) and immunoreagents: fluorescent-labeled antigen (tracer) and specific antibodies. The total time required for an assay is few seconds or minutes. The limit of detection for contaminants could be reached lower than typical Maximum Residue Limit (100 ng/ml or 100 ug/kg) after careful optimization antibodies and fluorescein-labeled tracers. The recent results for development of FPIAs for detection of contaminants in beverage will be discussed.