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Systematic bioinformatic analysis of the growing sequence and 3D-structural data representing evolutionarily distantly related proteins with diverse properties has a potential of becoming a particularly important tool in protein engineering and drug discovery, but represents a significant methodological challenge. We have developed the Mustguseal platform – a versatile on-line resource for knowledge-based protein design and modulation to facilitate experimental research, freely available at https://biokinet.belozersky.msu.ru/m-platform