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Detonation nanodiamonds were visualized in the volume of biological tissue by the method of coherent anti-Stokes light scattering (CARS) spectroscopy. To obtain the CARS signal, the system consisting of a pulsed Nd:YAG laser and a parametric light generator, which allows the radiation to be tuned in the range of 530-710 nm was used. CARS signals of carbon nanoparticles (carbon dots and detonation nanodiamonds) were obtained in aqueous suspensions and in the volume of biological tissue (egg white). The analysis of the obtained spectra made it possible to conclude the fundamental possibility of using CARS spectroscopy for visualizing nanoparticles in the tissue volume.