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This research focuses on landscapes studies with selected research area located in central Slovakia, Sitno area. The research purpose is to perform spatial analysis of the landscapes and patches within the landscapes, in order to assess, which parts of the selected study area have the highest environmental quality and value, for further environmental monitoring and management. This paper presents quantitative ecological assessment of the landscapes within the natural protected area located in Slovakia, central Europe. It contributes to the sustainable development and environmental management of the environmentally protected zones.The study area of Sitno Natura 2000 Sites is located in Slovakia, central Europe. This area belongs to the pan-European Natura 2000 network of ecologically protected areas. Natura 2000 sites are defined as pan-European reserved nature zones, which meet the requirements of high environmental criteria and belong to natural heritage of Europe. These landscapes are under strict environmental protection due to their high ecological value, unique species, rich biodiversity and other characteristics. Selected under Natura 2000 programme, habitats and endangered species of high ecological value and significance are considered as primary targets of nature conservation. Sitno Natura 2000 Sites include unique landscapes with rare and endangered species. Therefore, they have high environmental and natural value, as important part of the Slovakian and European natural heritage. At the same time, there are detected anthropogenic impacts on Sitno Natura 2000 Sites, caused by the uncontrolled tourism and other factors.