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Defects in synapsis and DSB repair, disturbance of nuclear architectonics during meiotic prophase I leads to checkpoint dependent arrest of meiosis described in many organisms. Here we describe spermatogenesis in allotriploid hybrid reptiles with multiple disturbances during prophase I. Triploids of a hybrid origin are not infrequent in reptiles. However, they have reproductive problems due to the limitations imposed by meiosis. Hybrid animals of different ploidy can be found in hybrid zones of rock lizards of genus Darevskia: triploids and even tetraploids. Triploid hybrids have been found at a high frequency in the hybrid zones. We studied immunostained preparation of prophase I nuclei and electron microscopy of spermatids in natural triploid hybrids males resulting from parthenogenetic D. unisexualis [2n=38 (36A+wZ)] and the males of parental species D. valentini [2n=38 (36A+ZZ)] and D. raddei nairensis [2n=38 (36A+ZZ)]. Three chromosome sets were found to occur in cell nuclei in the D. unisexualis × D. valentini [3n=57 (54A+wZZ)] hybrids, two originating from a parthenogenetic D.unisexualis female and one from the D. valentini male. Here we demonstrate distorted chromosome synapsis and incomplete double-strand breaks (DSB) repair in meiotic prophase I of triploid hybrid males. The competitive synapsis of three chromosome sets was studied in 329 cells and not a single spermatocyte I nucleus with complete chromosome synapsis was detected. We detected the evidence of crossing over in trivalents of homeologous chromosomes in D. unisexualis × D. valentini males. The number of mismatch repair foci (MLH1 foci) in the triploid hybrid was enough to pass through both meiotic divisions. Surprising finding of numerous abnormal mature spermatids in the testes of the studied hybrid was made. Thus we suppose that there is no strong selection against spermatocytes with incomplete synapsis, incomplete DSB repair or distorted chromosome desynapsis during prophase I in triploid hybrid males under study. Such abnormalities in mammals as a rule lead to arrest at prophase I stage.
№ | Имя | Описание | Имя файла | Размер | Добавлен |
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1. | Полный текст | тезисы устного доклада | ICC_2018_Spangenberg_abstract.pdf | 850,6 КБ | 21 июля 2021 [span] |