ИСТИНА

The state of human organism largely depends on blood microcirculation that depends on the microrheologic properties of red blood cells (RBCs), in particular, the RBC intrinsic properties of deformability and aggregation. Cardiovascular and metabolic diseases such as artherial hypertension (AH) and diabetes mellitus (DM) can lead to impairment of blood microrheology and microcirculation. The main goal of this work is to interrelate and assess the deformability and aggregation properties, forces of pair aggregation of RBCs drawn from patients suffering from AH, DM and from healthy donors using in vitro techniques of diffuse light scattering, laser diffractometry, laser trapping and manipulation and estimate the blood cappilary velocity by in vivo digital capillaroscopy. It was shown that in AH and DM patients, the ability of erythrocytes to deform is slightly reduced while the aggregation speed and forces of the cells interaction are significantly increased relative to the control group. The blood microcirculation in nailfold capillaries is impaired as well. We showed that the alterations of the parameters measured in vivo and in vitro for patients suffering from these diseases are interrelated. Good agreement between the results obtained with different techniques, and their applicability for the diagnostics of abnormalities of rheological properties of blood were demonstrated. The diffuse light scattering, laser diffractometry and laser trapping and manipulation are appropriate techniques for estimating the RBCs deformability and aggregation properties in whole blood samples and on the level of individual cells. These in vitro techniques allow to estimate disorders of blood micricirculation in pathologies. The study was supported by the Russian Foundation for Basic Research grant №19-52-51015 and Scientific School “Photonic and Quantum Technologies. Digital Medicine” of Lomonosov Moscow State University