ИСТИНА

DETECTION OF VETERINARY DRUGS BY FLUORESCENCE POLARIZATION IMMUNOASSAY S.A. Eremin1, N.V. Gasilova1, I.S.Nesterenko1, K. Aoki2, M. Tokeshi2 1M.V. Lomonosov Moscow State University; Moscow, Russia 2Hokkaido University, Sapporo, Japan The veterinary drugs are one of main contaminants in food and water samples. At this time it is growing problem. The samples must be controlled for toxic compounds in global scale with high-throughput screening (HTS) for many drugs. The current methods of assays like chromatography techniques and ELISA have several limitations for HTS. Immunoassays like ELISA is becoming an increasingly important method in measurement of drugs. Maybe the optimal method for food screening is immunoassays like Fluorescence Polarization Immunoassay (FPIA) [1]. The FPIA is a competitive homogeneous method which is characterized by good sensitivity, high specificity, considerable rapidity of several samples analysis. Now small-size equipment allows to work in any laboratory, even unequipped for this investigations and permits to make a procedure of screening less distant [2-3]. The FPIA based on the measurement of fluorescence polarization value for reaction mixture of sample (typically 10-50 µl) and immunoreagents: specific antibodies and fluorescent-labelled antigen (tracer). The total time required for an assay is few seconds or minutes. The recent results for development of FPIA for Sulfonamides, Chloramphenicol, Ampicillin, Streptomycin and other veterinary drugs will be discussed. The limit of detections were strongly depended from affinity of used antibodies and structures of applied tracers. Typically the LOD could be around 1-10 ng/ml and range of assay in 2-3 order. The FPIA method could be adapted for non-stop regime in flow system and could be dominant for veterinary drugs detections. Acknowledgements: The research was supported by Japan-Russia cooperation project “Development of microchip-based fluorescence polarization immunoassay of antibiotics in food samples” (RFBR 12-03-92105). [1] D. S. Smith and S. A. Eremin, Anal. Bioanal. Chem., 2008, 391, 1499-1507. [2] I.S. Nesterenko, M.A. Nokel, S.A. Eremin. Rus. J. Anal. Chem., 2009, 64, 435–444. [3] N.V. Gasilova, S.A. Eremin. Rus. J. Anal. Chem., 2010, 65, 255-259.