ИСТИНА

Extracellular vesicles (EVs) are cell-derived membrane vesicles, which are considered biocompatible carriers, but their practical use is restrained due to the lack of efficient production and isolation methods. The fabrication of EV mimetics has become more widespread in recent years. We focused our attention on nanovesicles obtained by treating cells with the actin destabilizing agent, cytochalasin B, and showed that these nanovesicles, named CINVs, were internalized by human cells more efficiently than EVs or other EV mimetics that we tested. The selectivity of CINV interaction with various human and mouse cell cultures as well as the effect of CINV on cell viability and proliferation were studied. The delivery of functional therapeutic nucleic acids into cells mediated by CINVs was also reported.