ИСТИНА

Aptasensors with Ag-nanostructured solid substrates in a rapid test version provide high sensitivity and a broad qualitative working concentration range of 104-108 virus particles in mL of influenza viruses. Whereas colloidal Ag nanoparticles provide a monotonous concentration dependency for a range of 105-109 VP/mL of influenza A or 5·104-2·106 TCID50/mL of SARS-CoV-2. Colloidal aptasensors do not require sophisticated equipment, this approach was chosen to create aptasensors for human cells and chemicals. Our aptasensor for human squamous carcinoma detected 103-105 cells/mL with a monotonous concentration dependence. As for small molecules, the aptasensor for adenosine monophosphate had a monotonous dependence in a range of 5-105 nM. Our results illuminate high promises for combination of SERS and aptamers in detection of supramolecular structures, biologicals and small molecules.