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Endocytosis is a complex process of transport into the cell and further distribution of soluble substances and macromolecules with the participation of the plasma membrane forming vesicles. Nowadays it is described about ten different types of endocytosis in animals according to the differences in the initial stages. Endocytosis in filamentous fungi is not studied enough. Investigation of endocytic mechanisms in fungi of such kind is a relevant and promising area for fungal cell biology, fundamental and applied mycology. Inhibitory analysis of endocytosis dynamics in plant pathogenic fungus Rhizoctonia solani with further tagging endocytic membrane with a fluorescent AM4-64 demonstrated that: a) actin cytoskeleton plays a key role in endocytosis; b) microtubular cytoskeleton plays an unusual role in endocytosis. It affects formation of primary vesicles that has not been shown for other organisms; c) inhibitors of different types of endocytosis (clathrin-dependent, dynamin-dependent and lipid-blast) decelerate or completely block it. Our results indicate the existence of conservative endocytic mechanisms in R. solani as well as in other eukaryotes. On the other hand, some features of endocytosis in R. solani suggest the presence of specific endocytic mechanisms in filamentous fungi. This work was supported by the Russian Foundation for Basic Research (RFBR) project № 16-04-00814.
№ | Имя | Описание | Имя файла | Размер | Добавлен |
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1. | Сборник тезисов FEMS 2017 | FEMS2017_abstracts-book_GS.pdf | 21,4 МБ | 23 мая 2018 [KudruavtsevaOA] |