ИСТИНА

Mesenchymal stem cells (MSC) are present in all organs and tissues, where they perform structural, trophic and regulating function and participate in tissue regeneration after injury. MSCs represent a heterogeneous population of cells that express different cell surface markers. MSCs also demonstrate various potential to differentiate into different cell lineages. It is known that only a very small proportion of these cells are indeed stem cells. The ratio between different cell types within this heterogeneous population varies depending on tissue source, isolation procedure and the conditions of cell culturing. The mechanisms that determine this heterogeneity in ontogenesis are still not well understood. We have analyzed the expression profile of MSCs isolated from human adipose tissue using the genome-wide screening method and RT-PCR and identified the expression of a number of hormone receptors (Serotonin receptors 1E, 2A, 2B, Adiponectin receptors-1, -2, Angiotensin II Receptor type 1, Bradykinin receptor, Thrombin receptor, Growth hormone receptor, Endothelin receptors, Leptin receptor, Oxytocin receptor, Parathyroid hormone receptor, α- and β- adrenoreceptors) and cytokines (CXCR1, TREM1, CCR7, CCR8, CXCR3, CXCR4, CCR1, CCRL2, CXCR6, CCR5, LTB4R, CCRL1, GPR81, CXCR5, CCR2, CCR4, IL8RA, CCR3, GPR31, CX3CR1, CCR6, C5AR1, CCR10). Immunofluorescent staining using antibodies against adrenergic receptors revealed that only a few cells express these receptors. The administration of hormones resulted in a rapid [Ca2+] upregulation in single cells confirming that the population of MSCs is heterogeneous not only morphologically , but also functionally. This study was supported by RSF grant 14-15-00439.