ИСТИНА

Adipose tissue is a special type of connective tissue that comprises stromal-vascular cells, committed preadipocytes, and mature adipocytes. It is particularly important to study the mechanisms of adipose tissue cell renewal, induction and regulation of stem/progenitor cell differentiation into mature adipocytes [1]. T-cadherin is a non-canonical representative of cadherins and a receptor for the adipose tissue hormone adiponectin, which is secreted by mature adipocytes. The role of T-cadherin in adipose tissue turnover and function is unknown. This investigation shows the content of T-cadherin by immunoblotting upon adipogenic induction at different stagesw using a 3T3-L1 mouse preadipocyte culture model. The level of T-cadherin changes during the induction of adipogenic differentiation and depends on the differentiation stage (4-7 days): the protein level in 3T3-L1 cells in the adipogenic medium was significantly higher throughout the experiment than in the cells in the control medium, reaching a maximum on the 10th day. The T-cadherin content in the cells in the control medium remained virtually unchanged throughout the study. The obtained data indicate the possible participation of T-cadherin in the processes of adipogenic differentiation both at early and later stages. Supported by the Russian Science Foundation grant no. 23-11-00205.