ИСТИНА

In regenerative and aesthetic medicine, artificially introduced polymers, products of hyaluronic acid (HA), are used to slow down the aging process (senescence). Since HA itself is an essential component of the intercellular matrix, it does not cause allergic reactions and inflammatory processes when it is introduced into the human body. When a HA-based drug is administered, its biodegradation process takes place by hydrolysis with the help of hyaluronidases, subsequently the smallest fragments penetrate into the cell by endocytosis, binding to the CD44 receptor. When CD44 interacts with HA, cyclin D expression is induced, which in turn affects the passage of the cell cycle and the transition of cells to senescence. However, the mechanism of fibroblast aging with the introduction of HA remains not fully disclosed.The purpose of this work is an experimental and theoretical study of the effect of HA on the aging process of human dermal fibroblast cell culture (DFC). The theoretical part of the study consisted in creating a mathematical model describing the degradation of HA by hyaluronidases, the capture of monomers through CD44-dependent endocytes, and a change in cyclin D expression. The validation of the model was carried out on the data of the MTT-test of DFC during cultivation in an environment with HA and its products, as well as on data on the collagen content determined by vertical PAAG electrophoresis. The aging of DPH was determined by the proportion of fibrocytes in the population when stained with hematoxylin-eosin.