ИСТИНА

One of the key challenges of the modern world is a transition from a society based on the ever-increasing use of fossil resources to a more sustainable one where renewable resources make a growing contribution to energy and material needs. Enzymatic conversion of agricultural and forestry stocks into a wide variety of fine and bulk chemicals, bioplastics, biofuels, pharmaceuticals etc. are gaining importance as starting material for future biotech products. Endo-1,4-b-glucanases (EG) play important role in cellulose biotransformation by converting non-crystalline polymer regions to cellooligosaccharides with low degree of polymerization. Those products can act as the inhibitors of enzymatic reaction catalyzed by endoglucanases. Purification and crystallization of Endoglucanase in native form and in the presence of cellobiose to determine the amino acids responsible for product binding.