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Two NOM samples from Irtyash and Tatysh lakes (South Urals, Russia) sorbed on XAD-8 resin according to the conventional technique were characterized using different analytical methods, such as: elemental analysis, size-exclusion chromatography (SEC), UV-vis spectrometry and C-13 NMR spectroscopy. Сomplex analytical method of NOM using different physicochemical techniques was demonstrated with an example of lake water dissolved organic matter.