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Influence of water molecules reversible adsorption on domain structure and domain wall structure in amorphous Fe-rich ferromagnetic samples is investigated by magneto-optical micro-magnetometer at room temperature. The investigated samples of composition Fe76,5Cu1Nb3Si13,5B6 were obtained by spinning technique. The new effect is found during investigation. Domain structure of the samples significantly changes in wacuum: instead one 180-degree domain wall several domain walls appear which are oriented with angle 45o to the long side of the sample. This effect is completely reversible. Another new effect is found during direct measuring domain wall width by magneto-optical method on the surface of the samples: domain wall width in vacuum is 35% bigger (13.5 µm) than in atmosphere of water vapor (10 µm).