|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ИНХС РАН |
||
Synthesis of triphenylphosphonium derivative of glutamic acid for further use in solid-phase peptide synthesisкурсовая работа (Бакалавр)
- Научный руководитель: Терещенков А.Г.
- Автор: Gao Yangziyan
- Тип: Бакалавр
- Организация, в которой проходила защита: МГУ имени М.В. Ломоносова
- Кафедра: Кафедра химической энзимологии
- Год защиты: 2024
- Курс: 3
- Аннотация: 1. A review of the available literature on triphenylphosphonium derivatives of peptides reveals that C-terminal TPP analogs offer numerous benefits, yet their synthesis via solely solid-phase methods remains elusive. 2. A three-step synthesis route was developed that made it possible to obtain a N-α-Fmoc-L-glutaminyl-(2-aminoethyl)triphenylphosphonium chloride from commercially available N-α-Fmoc, γ-tert-butyl L-glutamic acid and (2-bromoethyl)triphenylphosphonium bromide in a total yield of 13%. 3. The application of LC-MS, along with 1H, 13C, 19F, and 31P NMR techniques, enabled the confirmation of compound purity and the verification of its molecular structure. 4. Loading of synthesized conjugate on the RINK-amide resin allowed to obtain a polymer that can be further utilized in the synthesis of peptide derivatives containing the L-glutaminyl-(2-aminoethyl)triphenylphosphonium residue on their C-terminus.
- Добавил в систему: Терещенков Андрей Геннадьевич