Optitimization of a transient transformation method of Arabidopsis by Agrobacterium rhizogenes to insert nanosensors for pH measurements in plantsНИР

Соисполнители НИР

LIFE-KU Координатор

Источник финансирования НИР

Международная организация/программа, IAMONET-RU, Erasmus Mundus

Этапы НИР

# Сроки Название
1 6 января 2010 г.-2 апреля 2010 г. Optitimization of a transient transformation method of Arabidopsis by Agrobacterium rhizogenes to insert nanosensors for pH measurements in plants
Результаты этапа: We have implemented the protocol of A. rhizogenes mediated hypocotyl dip transformation of Arabidopsis seedlings. Composite plants were generated by inoculating shoots of Arabidopsis thaliana (L.) Heynh. ecotype Colombia-0 with Agrobacterium rhizogenes strain MSU440, containing plasmid with a GFP-mRFP1 pH nanosensor, developed previously or pRedRoot plasmid with the red fluorescence marker DsRED. Seedling age, sucrose content of the media and freshly prepared A. rhizogenes cultures were found to be critical steps affecting transformation efficiency.Imaging of DsRED1 or GFP/RFP was done using a Leica MZIII fluorescence stereomicroscope and a Leica SP2 confocal microscope. The most striking observation under high magnification was abnormal root hair morphology in Arabidopsis roots after A. rhizogenes transformation. We have observed twisted, branched and splitting root hairs in transgenic roots regardless of the containing plasmid.

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