Application of the Johnson–Kendall–Roberts model in AFM-based mechanical measurements on cells and gel

Efremov, Y.M.; Bagrov, D.V.; Kirpichnikov, M.P.; Shaitan, K.V.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 22 ноября 2015 г.

Авторы: Efremov Yu M., Bagrov D.V., Kirpichnikov M.P., Shaitan K.V.
Журнал: Colloids and Surfaces B: Biointerfaces
Том: 134
Год издания: 2015
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Первая страница: 131
Последняя страница: 139
DOI: 10.1016/j.colsurfb.2015.06.044
Аннотация: Abstract The force-distance curves (FCs) obtained by the atomic force microscope (AFM) with colloid probes contain information about both the viscoelastic properties and adhesion of a sample. Here, we processed both the approach and retraction parts of FCs obtained on polyacrylamide gels (in water or PBS) and Vero cells (in a culture medium). The Johnson–Kendall–Roberts model was applied to the retraction curves to account for the adhesion. The effects of loading rate, holding time and indentation depth on adhesion force and Young’s modulus, calculated from approach and retraction curves, were studied. It was shown that both bulk and local interfacial viscoelasticity can affect the observed approach-retraction hysteresis and measured parameters. The addition of 1% bovine serum albumin (BSA) decreased adhesion of the probe to the PAA gel surface, so interfacial viscoelasticity effects were diminished. On the contrary, the adhesiveness of Vero cells increased after BSA addition, indicating the complex nature of the cell-probe interaction.
Добавил в систему: Багров Дмитрий Владимирович

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Application of the Johnson–Kendall–Roberts model in AFM-based mechanical measurements on cells and gelстатья

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