Role of cytoskeleton network in anisosmotic volume changes of intact and permeabilized A549 cellsстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 26 декабря 2015 г.
Аннотация:Recently we found that cytoplasm of permeabilized mammalian cells behaves as a hydrogel displaying intrinsic
osmosensitivity. This study examined the role of microfilaments and microtubules in the regulation of hydrogel
osmosensitivity, volume-sensitive ion transporters, and their contribution to volume modulation of intact cells.
We found that intact and digitonin-permeabilized A549 cells displayed similar rate of shrinkage triggered by
hyperosmotic medium. It was significantly slowed-down in both cell preparations after disruption of actin
microfilaments by cytochalasin B, suggesting that rapid water release by intact cytoplasmic hydrogel contributes
to hyperosmotic shrinkage. In hyposmotic swelling experiments, disruption of microtubules by vinblastine attenuated
the maximal amplitude of swelling in intact cells and completely abolished it in permeabilized cells. The
swelling of intact cells also triggered ~10-fold elevation of furosemide-resistant 86Rb+ (K+) permeability and
the regulatory volume decrease (RVD), both ofwhich were abolished by Ba2+. Interestingly, RVD and K+ permeability
remained unaffected in cytocholasin/vinblastine treated cells demonstrating that cytoskeleton disruption
has no direct impact on Ba2+-sensitive K+-channels involved in RVD. Our results show, for the first time, that the
cytoskeleton network contributes directly to passive cell volume adjustments in anisosmotic media via the
modulation of the water retained by the cytoplasmic hydrogel.