|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ИНХС РАН |
||
Conformational changes in inter-alpha-trypsin inhibitor heavy chain 4 activate its tumor-specific activity in mice with B16 melanomaстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 19 сентября 2015 г.
- Авторы: Kormosh Natalya G., Davidova Tatyana V., Kopyltsov Vladimir N., Serebryakova Marina V., Kabieva Aigul O., Voyushin Konstantin E., Sitdikova Suriya M., Amandzholov Berick S., Kiselevskii Michail V., Donenko Fedor V.
- Журнал: Molecular Medicine Reports
- Том: 12
- Номер: 3
- Год издания: 2015
- Издательство: Spandidos Publications
- Местоположение издательства: Greece
- Первая страница: 4483
- Последняя страница: 4493
- DOI: 10.3892/mmr.2015.3961
- Аннотация: It is known that blood serum proteins of tumor-bearing mice display tumor-specific activity. However, to date, the nature of this activity has remained elusive, and no tumor-specific proteins have been detected in the blood serum of tumor-bearing animals compared with those in healthy animals. The present study postulated and investigated the hypothesis that the observed tumor-specific activity of the blood serum proteins is not associated with the appearance of novel serum proteins but with changes in the conformation of the existing ones. The present study showed conformational changes of two serum albumin proteins and inter-alpha-trypsin inhibitor heavy chainВ 4 (ITIH4) in mice with B16 melanoma compared to tumor-free mice, as determined by differences in the products of proteolysis by proteomic analysis following column chromatography. The differences in the conformation of serum albumin in mice with B16 melanoma and tumor‑free mice were accompanied by a change in the interaction of these molecules with the fatty acid spin probe 16-doxyl stearic acid. The differential conformation of ITIH4 in mice with B16 melanoma and that in tumor-free mice was accompanied by inhibition of tumor growth and increased life span. Analysis of the role of protease-anti-proteases (serpins) in the serum of tumor-bearing animals in tumor growth confirmed the hypothesis that tumor growth in the body is mediated, at least in part, via balancing of serpins.
- Добавил в систему: Серебрякова Марина Васильевна