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Restriction endonuclease SsoII with photoregulated activity - a "molecular gate" approachстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Thi Hien Le, Zatsepin T.S., Schierling B., Volkov E.M., Wende W., Pingoud A., Kubareva E.A., Oretskaya T.S.
- Журнал: Bioconjugate Chemistry
- Том: 22
- Номер: 7
- Год издания: 2011
- Издательство: American Chemical Society
- Местоположение издательства: United States
- Первая страница: 1366
- Последняя страница: 1373
- DOI: 10.1021/bc200063m
- Аннотация: A novel method for regulating the activity of homodimeric proteins--"molecular gate" approach--was proposed and its usefulness illustrated for the type II restriction endonuclease SsoII (R.SsoII) as a model. The "molecular gate" approach is based on the modification of R.SsoII with azobenzene derivatives, which allows regulating DNA binding and cleavage via illumination with light. R.SsoII variants with single cysteine residues introduced at selected positions were obtained and modified with maleimidoazobenzene derivatives. A twofold change in the enzymatic activity after illumination with light of wavelengths of 365 and 470 nm, respectively, was demonstrated when one or two molecules of azobenzene derivatives were attached to the R.SsoII at the entrance of or within the DNA-binding site.
- Добавил в систему: Зацепин Тимофей Сергеевич