Application of High Performance Liquid Chromatography-Tandem Mass Spectrometry for Detection of Melittin, a Characteristic Peptide of Bee Venomстатья
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Дата последнего поиска статьи во внешних источниках: 26 декабря 2015 г.
Аннотация:Bee venom is widely used for the manufacturing of cosmetics and pharmaceuticals. A procedure
for the detection of melittin, a characteristic component of bee venom, based on high performance liquid chromatography with tandem mass spectrometric detection is reported. Conditions of chromatography-mass spectrometric detection of melittin are selected, and a solid-phase extraction-based procedure for melittin isolation from preparations containing bee venom is developed. The procedure proposed is successfully tested on both raw venom samples and cosmetic and pharmaceutical preparations containing natural bee venom.