|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ИНХС РАН |
||
HMG-box factor SoxD/Sox15 and homeodomain-containing factor Xanf1/Hesx1 directly interact and regulate the expression of Xanf1/Hesx1 during early forebrain development in Xenopus laevisстатья
- Авторы: Martynova Natalia Y., Eroshkin Fedor M., Оrlov Eugeny E., Zaraisky Andrey G.
- Журнал: Gene
- Том: 638
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 52
- Последняя страница: 59
- DOI: 10.1016/j.gene.2017.09.024
- Аннотация: The homeodomain-containing transcription factor Anf (also known as Rpx/Hesx1 in mammals) plays an important role during the forebrain development in vertebrates. Here we demonstrate the ability of the Xenopus laevis Anf, Xanf1/Hesx1, to directly bind SRY-related HMG-box-containing transcription factor SoxD/Sox15 and to cooperate with the latter during regulating of the expression of Xanf1/Hesx1 own gene. As we have shown by GST pull-down, EMSA and the luciferase reporter assays, Xanf1/Hesx1 and SoxD/Sox15 bind the Xanf1/Hesx1 promoter region counteracting the inhibitory effect of Xanf1/Hesx1 alone. This finding explains how Xanf1/Hesx1 could escape the repressive activity of its own protein during early patterning of the forebrain rudiment.
- Добавил в систему: Зарайский Андрей Георгиевич