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Secretion of endo-1, 4-β- glucanases cellulase complex new extremely thermophilic anaerobic bacteria Anaerocellum thermophilum gen. nov., sp. novстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 20 апреля 2016 г.
- Авторы: Tikhomirov D.F., Stolbova V.V., Svetlichnyi V.A., Klesov A.A., Zavarzin G.A.
- Журнал: Prikladnaya Biokhimiya i Mikrobiologiya
- Том: 26
- Номер: 2
- Год издания: 1990
- Издательство: Izdatel'stva Nauka
- Местоположение издательства: Russian Federation
- Первая страница: 252
- Последняя страница: 259
- Аннотация: Conditions of the endo-1,4-.beta.-glucanase biosynthesis in an extremely thermophilic strictly anaerobic non-sporulating bacterium, strain Z 1320, were studied. The highest level of endoglucanase activity was detected, when the bacterium was cultivated at 70-75.degree. C, pH 7.0 on a medium with insoluble substrates-microcrystalline cellulose and filter paper; a lower level was observed in case of cellobiose, glucose and glucose 6-phosphate. Organic additive (vitamins, yeasts and maize extracts) repressed the endoglucanase biosynthesis. On 0.1-1.0% cellulose as a sole organic carbon source, the maximal rate of endoglucanase formation was observed up to 4 days of cultivation and coincided with the exponential phase of biomass accumulation. To this moment, from 80 to 19% of the substrate was solubilized (0.8-1.9 g/L); the final endoglucanase level being 0.017-0.013 U/ml, respectively, 3.3 and 40% of endoglucanase was adsorbed on 0.1% and 1% cellulose of the growth medium, respectively, and less than 1% of the total enzyme was located on the cell surface and inside the cells.
- Добавил в систему: Столбова Валерия Владимировна