Preparation and Application of a BODIPY Labeled Probe for a Real Time Polymerase Chain Reactionстатья
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Дата последнего поиска статьи во внешних источниках: 2 декабря 2015 г.
Аннотация:A BODIPY based (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene, TMB) green fluorescent probe for quantitative realtime polymerase chain reaction (qPCR) was synthesized by azidealkyne cycloaddition. Comparative studies with the analogous fluorescein-based probe were carried out. We demonstrated that fluorescent probes with the TMB fluorophore can be used in qPCR experiments.