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A fluorescence polarization immunoassay method for detection of the bisphenol A residue in environmental water samples based on a monoclonal antibody and 4′-(aminomethyl)fluoresceinстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 3 декабря 2015 г.
- Авторы: Huang Pinong, Zhao Suqing, Eremin Sergei A., Zheng Shengwu, Lai Dan, Chen Yingshan, Guo Bin
- Журнал: Analytical Methods
- Том: 7
- Номер: 10
- Год издания: 2015
- Издательство: Royal Society of Chemistry
- Местоположение издательства: United Kingdom
- Первая страница: 4246
- Последняя страница: 4251
- DOI: 10.1039/c5ay00818b
- Аннотация: Based on a sensitive monoclonal antibody against bisphenol A (BPA) and a new tracer named BVA–AMF, a homogeneous fluorescence polarization immunoassay (FPIA) was developed and applied in the determination of bisphenol A in environmental water samples. BVA was selected as the hapten to couple with bovine serum albumin and the conjugate was used as the immunogen for the monoclonal antibody production. Three fluorescein-labeled BVA tracers with different structures (BVA–AMF, BVA–EDF, and BVA–lysFITC) were synthesized. Under the same optimal conditions, BVA–AMF showed the highest sensitivity for FPIA and the detection of BPA exhibited a limit of detection of 5.60 ng mL_1, an IC50 of 140 ng mL_1 and a dynamic range of 11.32–904.21 ng mL_1 approximately. In this assay, the monoclonal antibody against BPA showed high specificity towards BPA with less than 0.15% cross-reactivity against several other similar compounds. Four different kinds of water samples were analyzed, with recoveries being 87.91–114.28%. The detection standard curve of BPA exhibited a good linearity (R2 ј 0.9913, n ј 3). Compared with ELISA and HPLC methods, FPIA showed reliability and a high correlation with ELISA of 0.9964 and HPLC of 0.9971. The proposed immunoassay technique is suitable for detection of BPA in authentic environmental water samples.
- Добавил в систему: Еремин Сергей Александрович