Chemiluminescent Determination of MicroRNA-141 using Target-Dependent Activation of the Peroxidase-Mimicking DNAzymeстатья
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Дата последнего поиска статьи во внешних источниках: 25 апреля 2019 г.
Аннотация:Chemiluminescent method for microRNA-141 (miRNA-141) detection based on target-dependent activation of peroxidase-mimicking DNAzyme (PMDNAzyme) was developed. The structure of the probes was optimized, which allowed constructing a sensitive method for miRNA-141 detection. Under favorable conditions the detection limit value and the linear range were shown to be 100 pM and 0.1-50 nM, respectively. The sensitivity of the assay was 270 000 nM-1. The values of coefficient of variation measured within the working range varied less than 2%, which indicates a high precision of the proposed method.