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The expression of matryoshka gene encoding a homologue of the Kunitz peptidase inhibitor is regulated both at the level of transcription and translationстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 6 декабря 2018 г.
- Авторы: Sheshukova Еkaterina V., Ershova N.M., Komarova T.V., Bronstein A.M., Dorokhov Y.L.
- Журнал: Biochemistry (Moscow)
- Том: 83
- Номер: 10
- Год издания: 2018
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 1255
- Последняя страница: 1262
- DOI: 10.1134/S0006297918100103
- Аннотация: —The gene for Kunitz peptidase inhibitorlike protein (KPILP) contains nested alternative open reading frame (aORF) that controls expression of the maternal mRNA. The content of NbKPILP mRNA in intact leaves of Nicotiana ben thamiana plant is low but increases significantly upon extended dark exposure or when foreign nucleic acid is overexpressed in the cells. The NbKPILP gene promoter along with the expressed nested aORF are likely to play an important role in maintaining the levels of NbKPILP mRNA. To elucidate the role of NbKPILP promoter, we isolated a fragment of N. ben thamiana chromosomal DNA upstream of the NbKPILP transcription start, sequenced it, and created constructs in which reporter E. coli uidA gene coding for βDglucuronidase (GUS) was placed under control of the NbKPILP promoter. By assessing the efficacy of uidA mRNA synthesis directed by the NbKPILP promoter and 35S promoter of the cauliflower mosaic virus in a transient expression system, we showed that the levels of GUS accumulation were comparable for both promoters. Prolonged incubation of the agroinjected plants in the darkness stimulated accumulation of the uidA mRNA direct ed by the NbKPILP promoter. Our experiments indicate that along with regulation at the transcriptional level, expression of NbKPILP mRNA can be affected by expression of the nested aORF controlled by the polypurine block (PPB) located upstream of its start codon, since introduction of mutations in the PPB resulted in significant accumulation of the NbKPILP mRNA. Nucleotide replacement in the aORF start codon led to the drastic increase in the amounts of NbKPILP mRNA and its protein product. DOI:10.1134/S0006297918100103
- Добавил в систему: Дорохов Юрий Леонидович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | bcm_1562_1571_Sheshukova.pdf | bcm_1562_1571_Sheshukova.pdf | 366,9 КБ | 28 августа 2018 [Dorokhov] | |
| 2. | BM18-120_decision-3_a.pdf | BM18-120_decision-3_a.pdf | 130,1 КБ | 16 июля 2018 [Dorokhov] |