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Human embryonic stem cell–derived cardiomyocytes restore function in infarcted hearts of non-human primatesстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 10 августа 2018 г.
- Авторы: Liu YW, Chen B., Yang X., Fugate JA, Kalucki FA, Futakuchi-Tsuchida A., Couture L., Vogel KW, Astley CA, Baldessari A., OgleJ Don CW, Steinberg ZL, Seslar SP, Tuck SA, Tsuchida H., Naumova AV, Dupras SK, Lyu MS, Lee J., Hailey DW, Reinecke H., Pabon L., Fryer BH, MacLellan WR, Thies RS, Murry CE
- Журнал: Nature Biotechnology
- Том: 36
- Номер: 7
- Год издания: 2018
- Издательство: Nature Publishing Group
- Местоположение издательства: United Kingdom
- Первая страница: 597
- Последняя страница: 605
- DOI: 10.1038/nbt.4162
- Аннотация: Pluripotent stem cell–derived cardiomyocyte grafts can remuscularize substantial amounts of infarcted myocardium and beat in synchrony with the heart, but in some settings cause ventricular arrhythmias. It is unknown whether human cardiomyocytes can restore cardiac function in a physiologically relevant large animal model. Here we show that transplantation of ∼750 million cryopreserved human embryonic stem cell–derived cardiomyocytes (hESC-CMs) enhances cardiac function in macaque monkeys with large myocardial infarctions. One month after hESC-CM transplantation, global left ventricular ejection fraction improved 10.6 ± 0.9% vs. 2.5 ± 0.8% in controls, and by 3 months there was an additional 12.4% improvement in treated vs. a 3.5% decline in controls. Grafts averaged 11.6% of infarct size, formed electromechanical junctions with the host heart, and by 3 months contained ∼99% ventricular myocytes. A subset of animals experienced graft-associated ventricular arrhythmias, shown by electrical mapping to originate from a point-source acting as an ectopic pacemaker. Our data demonstrate that remuscularization of the infarcted macaque heart with human myocardium provides durable improvement in left ventricular function.
- Добавил в систему: Наумова Анна Владимировна