Effect of Molecular Weight and Degree of Acetylation on Adjuvantive Properties of Chitosan Derivatives

Markushin, S.G.; Akopova, I.I.; Blagodatskikh, I.V.; Kulikov, S.N.; Bezrodnykh, E.A.; Muranov, A.V.; Yamskov, I.A.; Tikhonov, V.E.

Авторы: Markushin S.G., Akopova I.I., Blagodatskikh I.V., Kulikov S.N., Bezrodnykh E.A., Muranov A.V., Yamskov I.A., Tikhonov V.E.
Журнал: Applied Biochemistry and Microbiology
Том: 54
Номер: 5
Год издания: 2018
Издательство: Maik Nauka/Interperiodica Publishing
Местоположение издательства: Russian Federation
Первая страница: 512
Последняя страница: 517
DOI: 10.1134/S0003683818050149
Аннотация: The hemostatic and immunostimulating activity and cytotoxicity were determined for a number of chitosans differing in molecular weight (from 3 to 510 kDa) and degree of acetylation (from 1 to 25 mol%) that were used as adjuvants in inactivated poliomyelitic, influenza, and live influenza vaccines. It has been shown that the hemostatic activity of chitosan increased sharply with an increase in its molecular weight. In oligochitosan with a molecular weight of <16 kDa, it was smaller by a factor of 15–100 than in chitosan with a molecular weight of 20–510 kDa. The level of increase in the immunogenicity of vaccines containing oli- gochitosan as adjuvants was not lower than that for the vaccine including high-molecular chitosan. However, the immunostimulatory activity of oligochitosan depended on the degree of acetylation, reaching a maximum value at 6 mol%. It was shown that all oligochitosans and chitosans with a molecular mass below ~50 kDa showed almost no cytotoxicity at a concentration of ≤2.5 mg/mL, which enable their use as adjuvants for inactivated and live vaccines at the optimal ratio of molecular weight to the degree of acetylation.
Добавил в систему: Тихонов Владимир Евгеньевич

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Effect of Molecular Weight and Degree of Acetylation on Adjuvantive Properties of Chitosan Derivativesстатья

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