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Influence of NO and [Ca2+]o on [Ca2+]i homeostasis in rat ventricular cardiomyocytesстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 ноября 2020 г.
- Авторы: Mitrokhin Vadim, Mladenov Mitko, Gorbacheva Lyubov, Babkina Irina, Lovchikova Irina, Kazanski Viktor, Kamkin Andre
- Журнал: Biotechnology and Biotechnological Equipment
- Том: 32
- Номер: 5
- Год издания: 2018
- Издательство: Diagnosis Press Ltd.
- Местоположение издательства: Bulgaria
- Первая страница: 1338
- Последняя страница: 1343
- DOI: 10.1080/13102818.2018.1488621
- Аннотация: In this study, we explored the effect of NO-induced changes in [Ca2þ]i homeostasis in rat ventricular cardiomyocytes through variation in extracellular Ca2þ and application of SNAP (Snitroso-N-acetyl-D,L-penicillamine) as an NO-donor. The Fura-2 signal dynamics and phalloidin intensity profile of z-disks in rat cardiomyocytes served as endpoints to describe the mechanisms involved in the control of the intracellular Ca2þ levels, depending on the kinetics of distribution of the SNAP-produced NO. The results demonstrated that SNAP caused small phalloidin intensity profile changes between the z-lines in the presence of [Ca2þ]o. However, in the absence of [Ca2þ]o, SNAP in a concentration of 300 lmol/L induced a significant decrease in the distance between z-lines. This SNAP-induced decrease was reflected as a decrease in the phalloidin fluorescence intensity in the middle of the sarcomere, due to the preferential imaging of greater fluorescence in the bulk of the thin filaments. Based on the Ca2þ fluorescence intensity profile, we could suggest that intracellular Ca2þ is mainly affected by the mechanisms of Ca2þ outflow, rather than the mechanisms of Ca2þ inflow in the presence of NO. Actually, through variation in the electrochemical gradient of Ca2þ, we induced mechanisms of faster/slower cytosolic Ca2þ emptying. The obtained data showed that the delay in the cytosolic Ca2þ emptying in the presence of [Ca2þ]o is due to the increased electrochemical gradient of Ca2þ.
- Добавил в систему: Горбачева Любовь Руфэльевна