Activated Protein C (APC) via PAR1 Protects Neurons from Death in the Model of Neuroinflammation in vitro

Авторы: Babkina I., Gorbacheva L., Strukova S., Pinelis V., Reiser G.
Журнал: Research and Practice in Thrombosis and Haemostasis
Том: 1
Номер: S1
Год издания: 2017
Издательство: John Wiley & Sons
Местоположение издательства: Hoboken, NJ, United States
Первая страница: 486
Последняя страница: 486
DOI: 10.1002/rth2.12012
Аннотация: Using a model of the neuroinflammation we shown that APCand AP9 protect neurons from death induced by LPS-activated mastcells. The pretreatment activated mast cells with APC(10nM) or AP9(10μM) decreased the number of apoptotic neurons to 1,9 and 1,8fold, respectively. Receptor mechanism of APC and AP9 action wasidentified by the blocking of PAR1 on mast cells or neurons by anti-PAR1 antibodies or PAR1-inhibitor SCH 79797. We shown that theblockage of PAR1 abolished protective effects of APC and AP9 andincreased apoptotic cells at 1,4 and 2,8 fold, respectively. Thus, PAR1was required for the protective effect of the APC and AP9.Thus, at the first time we have demonstrated the neuroprotectiveeffects of APC and AP9 in the neuroinflammation. Theprotective effects of APC and AP9 on mast cells and neurons are thesame and realized through PAR1. Thus, the new peptide AP9 is a functionalanalog of APC.
Добавил в систему: Горбачева Любовь Руфэльевна

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