Molecular Requirements for the Transition from Lateral to End-on Microtubule Binding and Dynamic Coupling. Ekaterina L Grishchuk, Manas Chakraborty, Anatoly V Zaytsev, Maxim Godzi, Ekaterina Tarasovetc, Ana C Figueiredo, Fazly I Ataullakhanovстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 22 февраля 2019 г.
Аннотация:Accurate chromosome segregation relies on the ill-understood ability of kinetochores to convert their lateral microtubule attachment into the microtubule plus-end association, capable of the processive motion with tubulin dynamics. Here we report that this transition can be recapitulated in vitro using only two components: the plus end-directed kinesin CENP-E and the microtubule wall-binding Ndc80 protein. CENP-E's primary role is to establish the end configurations for Ndc80, while Ndc80 mediates the maintenance of end attachment.