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Ultrahigh-throughput functional profiling of microbiota communitiesстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 16 января 2019 г.
- Авторы: Terekhov Stanislav S., Smirnov Ivan V., Malakhova Maja V., Samoilov Andrei E., Manolov Alexander I., Nazarov Anton S., Danilov Dmitry V., Dubiley Svetlana A., Osterman Ilya A., Rubtsova Maria P., Kostryukova Elena S., Ziganshin Rustam H., Kornienko Maria A., Vanyushkina Anna A., Bukato Olga N., Ilina Elena N., Vlasov Valentin V., Severinov Konstantin V., Gabibov Alexander G., Altman Sidney
- Журнал: Proceedings of the National Academy of Sciences of the United States of America
- Том: 115
- Номер: 38
- Год издания: 2018
- Издательство: National Academy of Sciences
- Местоположение издательства: United States
- Первая страница: 9551
- Последняя страница: 9556
- DOI: 10.1073/pnas.1811250115
- Аннотация: Analyzing complex microbial communities is the milestone of modern microbiology, calling for “deep functional profiling” techniques. While next generation sequencing revolutionized our understanding of microbiota communities, we still lack high-throughput technologies to precisely determine their functionality. Here we show how cultivation of individual bacteria inside droplets of microfluidic double water-in-oil-in-water emulsion enables us to isolate the clones with a desired activity. This approach allows us not only to select the potent antibiotic producer but also to discover a distinct mechanism of self-resistance as well as assess its efficiency on entire microbiomes. The outcome of this methodology shows that it could be effectively transferred to numerous applications in microbiology and biotechnology.
- Добавил в систему: Остерман Илья Андреевич