Mitochondria-targeted antioxidant SkQ1 improves corneal healing after UV-induced damage in rabbitsстатьяТезисы
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Web of Science
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Дата последнего поиска статьи во внешних источниках: 22 февраля 2019 г.
Аннотация:Cornea absorbs most of daily ultraviolet (UV) light. An excess of UV damages results in not only keratopathy and cataract but also maculopathy. Previously, it was demonstrated UV exposure are associated with mitochondrial dysfunction and oxidative stress in cultured corneal epithelial cells in vitro. In this work, the feasibility of mitochondria targeted antioxidant SkQ1 based therapy for enhancement of corneal regeneration after UV irradiation was demonstrated in vivo. A model of corneal damage caused by UV radiation was developed. It was demonstrated that 312 nm light irradiation of the cornea triggers the development of corneal lesions in rabbits. In particular, loss of epithelium, stromal edema and apoptosis of keratocytes and endothelium cells were observed. The stepwise mechanism of corneal regeneration was characterized. It involves epithelium and endothelium regrowth and activation of healthy keratocytes. Regeneration takes place simultaneously in all corneal layers and it becomes completed by day 7. It was emphasized that multiple apoptosis of stromal keratocytes can be associated with UVinduced oxidative stress, which manifested in the cornea as elevation of MDA content. The feasibility of SkQ1 based antioxidant therapy for enhancement of corneal regeneration after UV irradiation was demonstrated. The therapy prevents the loss of stromal keratocytes and othercorneal cells, facilitates reepithelialization of damaged tissue area and enhances stromal remodeling. In general, clinical and histological data indicate that the treatment accelerates corneal healing by 3 days. According to biochemical studies,the instillations of SkQ1 also suppresses oxidative stress in the cornea. In conclusion, our data suggest that the mitochondria targeted antioxidant SkQ1 is effective in improving regenerative phases of corneal healing after UV damage.